Low-Cost Methods for Molecular Characterization of Mutant Plants
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Low-Cost Methods for Molecular Characterization of Mutant Plants
Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Laport, Robert G.; Jankowicz-Cieslak, Joanna; Till, Bradley J.; Hofinger, Bernhard J.; Beshir, Mayada M.; Huynh, Owen A.
Springer International Publishing AG
10/2016
38
Mole
Inglês
9783319386676
15 a 20 dias
97
Descrição não disponível.
?Introduction.- Health and Safety Considerations.- Sample Collection and Storage.- Low-Cost DNA Extraction.- PCR Amplification for Low-Cost Mutation Discovery.- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples.- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants.- Example Data.- Conclusions.
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Crop improvement;DNA extraction;Induced mutations;Low-cost assays;Mutant germplasm characterization;Nucleotide variation in plant genomes;PCR amplification
?Introduction.- Health and Safety Considerations.- Sample Collection and Storage.- Low-Cost DNA Extraction.- PCR Amplification for Low-Cost Mutation Discovery.- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples.- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants.- Example Data.- Conclusions.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
